Chromosomal sublocalization of the 2;13 translocation breakpoint in alveolar rhabdomyosarcoma

Genes Chromosomes Cancer. 1992 Apr;4(3):241-9. doi: 10.1002/gcc.2870040309.


A characteristic balanced reciprocal chromosomal translocation [t(2;13)(q35;q14)] has been identified in more than 50% of alveolar rhabdomyosarcomas. As the first step in characterization of the genes involved in this translocation, we constructed somatic cell hybrids that retained either the derivative chromosome 2 or the derivative chromosome 13 without a normal chromosome 13 homologue. Ten linked DNA probes known to be located within bands 13q13-q14 were mapped relative to the breakpoint on chromosome 13, allowing localization of the breakpoint region between two loci separated by 5.5 cM. A long-range restriction map extending approximately 2,300 kb around these loci failed to provide evidence of rearrangement. Additionally, we confirmed that the FMS-like tyrosine kinase gene (FLT), previously localized to 13q12 by in situ hybridization, is located proximal to the breakpoint, and we demonstrated that FLT is not a target for disruption by this tumor-specific translocation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Cell Line
  • Chromosomes, Human, Pair 13
  • Chromosomes, Human, Pair 2*
  • DNA / analysis
  • DNA Probes
  • Electrophoresis, Agar Gel
  • Genetic Linkage
  • Humans
  • Mandibular Neoplasms / genetics*
  • Nucleic Acid Hybridization
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogenes
  • Restriction Mapping
  • Rhabdomyosarcoma / genetics*
  • Translocation, Genetic*
  • Vascular Endothelial Growth Factor Receptor-1


  • DNA Probes
  • Proto-Oncogene Proteins
  • DNA
  • Vascular Endothelial Growth Factor Receptor-1