Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures

Biochemistry. 1992 Sep 15;31(36):8406-14. doi: 10.1021/bi00151a003.


A model for the solution structure of an RNA tetraplex, (rUGGGGU)4, has been obtained by two-dimensional NMR spectroscopy and molecular dynamics. The molecule is parallel stranded and Hoogsteen base-paired in 50 mM KCl, and it is so stable that three of its six imino protons have exchange half-lives measured in days at 40 degrees C. The tetraplex is stabilized by base stacking and by the hydrogen bonds in four G quartets and at least one U quartet. This is the first indication of the existence of U-quartet structures of which we are aware.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Models, Chemical
  • Nucleic Acid Conformation
  • Oligoribonucleotides / chemistry*
  • RNA / chemistry
  • RNA / ultrastructure
  • Solutions


  • Oligoribonucleotides
  • Solutions
  • RNA