Developmental expression of voltage-sensitive K+ channels in mouse skeletal muscle and C2C12 cells

FEBS Lett. 1992 Sep 28;310(2):162-6. doi: 10.1016/0014-5793(92)81320-l.


The developmental expression of voltage-sensitive K+ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C2C12 has been used to study expression of voltage-sensitive K+ channels during in vitro myotube differentiation. Different voltage-sensitive K+ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K+ channel of IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Brain / growth & development
  • Brain / metabolism
  • Cell Differentiation
  • Cells, Cultured
  • DNA
  • DNA Probes
  • Mice
  • Muscle Development
  • Muscles / metabolism*
  • Polymerase Chain Reaction
  • Potassium Channels / genetics
  • Potassium Channels / metabolism*
  • RNA / metabolism


  • DNA Probes
  • Potassium Channels
  • RNA
  • DNA