Detection of endothelin-1 mRNA by RT-PCR in isolated rat renal tubules

Biochem Biophys Res Commun. 1992 Oct 15;188(1):108-13. doi: 10.1016/0006-291x(92)92356-3.


A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5' and 3' primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • DNA / genetics
  • DNA / isolation & purification
  • Endothelins / genetics*
  • Kidney Cortex / physiology
  • Kidney Glomerulus / physiology
  • Kidney Medulla / physiology
  • Kidney Tubules / physiology*
  • Kidney Tubules, Collecting / physiology
  • Male
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis*
  • RNA, Messenger / genetics
  • RNA-Directed DNA Polymerase
  • Rats
  • Rats, Wistar


  • Endothelins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • RNA-Directed DNA Polymerase