Abstract
The insulin-like growth factors, IGF-I and IGF-II, circulate in both humans and rats as part of a 125-150 kDa complex comprising IGFs, the IGF binding protein IGFBP-3, and an acid-labile subunit. Clones encoding rat acid-labile subunit have been isolated from a rat liver cDNA library probed with a human acid-labile subunit cDNA. Two overlapping clones encode a leucine-rich protein of 576 amino acids preceded by a 27-residue signal sequence, with 78% homology to the human acid-labile subunit. Northern analysis of mRNA from adult rat brain, kidney, heart, lung, spleen, muscle and liver shows a major species of about 4.4 kb and minor bands of about 2 kb, 1.4 kb and 1 kb. The tissue distribution of this protein may therefore be wider than previously recognized.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Carrier Proteins / genetics*
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Cloning, Molecular / methods
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DNA / genetics
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Gene Library
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Humans
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Insulin-Like Growth Factor Binding Proteins
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Liver / physiology
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Macromolecular Substances
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Molecular Sequence Data
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Molecular Weight
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Organ Specificity
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RNA, Messenger / genetics*
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RNA, Messenger / isolation & purification
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Rats
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Rats, Inbred WF
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Repetitive Sequences, Nucleic Acid
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Sequence Homology, Nucleic Acid
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Somatomedins / metabolism
Substances
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Carrier Proteins
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Insulin-Like Growth Factor Binding Proteins
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Macromolecular Substances
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RNA, Messenger
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Somatomedins
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DNA
Associated data
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GENBANK/D10916
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GENBANK/D10917
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GENBANK/D10918
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GENBANK/D10919
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GENBANK/D10920
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GENBANK/D12679
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GENBANK/S41680
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GENBANK/S41731
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GENBANK/S44053
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GENBANK/S46785