Molecular cloning of the acid-labile subunit of the rat insulin-like growth factor binding protein complex

Biochem Biophys Res Commun. 1992 Oct 15;188(1):304-9. doi: 10.1016/0006-291x(92)92385-b.

Abstract

The insulin-like growth factors, IGF-I and IGF-II, circulate in both humans and rats as part of a 125-150 kDa complex comprising IGFs, the IGF binding protein IGFBP-3, and an acid-labile subunit. Clones encoding rat acid-labile subunit have been isolated from a rat liver cDNA library probed with a human acid-labile subunit cDNA. Two overlapping clones encode a leucine-rich protein of 576 amino acids preceded by a 27-residue signal sequence, with 78% homology to the human acid-labile subunit. Northern analysis of mRNA from adult rat brain, kidney, heart, lung, spleen, muscle and liver shows a major species of about 4.4 kb and minor bands of about 2 kb, 1.4 kb and 1 kb. The tissue distribution of this protein may therefore be wider than previously recognized.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Cloning, Molecular / methods
  • DNA / genetics
  • Gene Library
  • Humans
  • Insulin-Like Growth Factor Binding Proteins
  • Liver / physiology
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Weight
  • Organ Specificity
  • RNA, Messenger / genetics*
  • RNA, Messenger / isolation & purification
  • Rats
  • Rats, Inbred WF
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid
  • Somatomedins / metabolism

Substances

  • Carrier Proteins
  • Insulin-Like Growth Factor Binding Proteins
  • Macromolecular Substances
  • RNA, Messenger
  • Somatomedins
  • DNA

Associated data

  • GENBANK/D10916
  • GENBANK/D10917
  • GENBANK/D10918
  • GENBANK/D10919
  • GENBANK/D10920
  • GENBANK/D12679
  • GENBANK/S41680
  • GENBANK/S41731
  • GENBANK/S44053
  • GENBANK/S46785