S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart

Biochemistry. 1992 Oct 27;31(42):10258-64. doi: 10.1021/bi00157a012.


Elevated levels of intracellular calcium are a major cause of myocardial dysfunction. To find possible mediators of the deregulated calcium we searched for EF-hand calcium-binding proteins of the S100 family. By PCR technology we identified three members of the S100 protein family (S100 alpha, CACY, and CAPL) in the human heart. We cloned the corresponding cDNAs and examined their expression levels in various human tissues by Northern blot analysis. All three proteins are expressed at high levels in the human heart. Whereas CACY and CAPL mRNAs are expressed ubiquitously, S100 alpha mRNA is restricted to heart, skeletal muscle, and brain. Interestingly, the expression pattern of S100 alpha, CACY, and CAPL in human tissues differs significantly from that in rodent tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Calcium-Binding Proteins / genetics*
  • Cattle
  • Cell Cycle Proteins*
  • Cloning, Molecular
  • Gene Expression
  • Gene Library
  • Heart Ventricles
  • Humans
  • Molecular Sequence Data
  • Myocardium / metabolism*
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / genetics
  • Rats
  • Restriction Mapping
  • S100 Calcium Binding Protein A6
  • S100 Calcium-Binding Protein A4
  • S100 Proteins*
  • Sequence Homology, Amino Acid


  • Calcium-Binding Proteins
  • Cell Cycle Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • S100 Calcium Binding Protein A6
  • S100 Calcium-Binding Protein A4
  • S100 Proteins
  • S100A1 protein
  • S100A4 protein, Bos taurus
  • S100a4 protein, rat
  • S100a6 protein, rat
  • S100A6 protein, human
  • S100A4 protein, human
  • RNA

Associated data

  • GENBANK/D10993
  • GENBANK/D10994
  • GENBANK/M80563
  • GENBANK/M86481
  • GENBANK/S46845
  • GENBANK/S46846
  • GENBANK/S46849
  • GENBANK/X58079
  • GENBANK/X65732
  • GENBANK/X65733
  • GENBANK/X65734