Cloning and sequencing of a rat type II activin receptor

FEBS Lett. 1992 Nov 2;312(1):53-6. doi: 10.1016/0014-5793(92)81408-e.

Abstract

A full-length cDNA for a rat type II activin receptor was cloned by hybridization from a rat ovary cDNA library. The deduced amino acid sequence (513 residues) containing a single membrane-spanning domain and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 99.8% and 99.4% identical in the coding region with the previously cloned mouse and human type II activin receptor, and only 66.7% identical in the coding region with the previously cloned rat type IIB activin receptor. We examined the effect of PMSG-hCG on the mRNA level of type II activin receptor in immature rat ovaries. Northern blot analysis of ovarian RNA revealed two mRNAs (3.0 kb and 6.0 kb).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activin Receptors
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chorionic Gonadotropin / pharmacology
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Gonadotropins, Equine / pharmacology
  • Humans
  • Molecular Sequence Data
  • Ovary / drug effects
  • Ovary / physiology
  • Poly A / genetics
  • Protein-Serine-Threonine Kinases / genetics*
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Rats
  • Rats, Wistar
  • Receptors, Cell Surface / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Chorionic Gonadotropin
  • Gonadotropins, Equine
  • RNA, Messenger
  • Receptors, Cell Surface
  • Poly A
  • RNA
  • DNA
  • Protein-Serine-Threonine Kinases
  • Activin Receptors

Associated data

  • GENBANK/S39996
  • GENBANK/S40001
  • GENBANK/S40008
  • GENBANK/S40009
  • GENBANK/S40010
  • GENBANK/S48190
  • GENBANK/X65120
  • GENBANK/X72578
  • GENBANK/X72579
  • GENBANK/X72580