Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel

FEBS Lett. 1992 Nov 16;313(1):34-8. doi: 10.1016/0014-5793(92)81178-o.


The primary structure of a novel subunit of the mouse NMDA (N-methyl-D-aspartate) receptor channel, designated epsilon 4, has been revealed by cloning and sequencing the cDNA. The epsilon 4 subunit shares high amino acid sequence identity with the epsilon 1, epsilon 2 and epsilon 3 subunits of the mouse NMDA receptor channel, thus constituting the epsilon subfamily of the glutamate receptor channel. Expression from cloned cDNAs of the epsilon 4 subunit together with the zeta 1 subunit in Xenopus oocytes yields functional NMDA receptor channels. The epsilon 4/zeta 1 heteromeric channel exhibits high apparent affinities for agonists and low sensitivities to competitive antagonists. The epsilon 4 subunit is thus distinct in functional properties from the epsilon 1, epsilon 2 and epsilon 3 subunits, and contributes further diversity of the NMDA receptor channel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • Gene Expression
  • Ion Channels / genetics*
  • Membrane Potentials
  • Mice
  • Molecular Sequence Data
  • Receptors, N-Methyl-D-Aspartate / genetics*
  • Sequence Homology, Amino Acid
  • Xenopus laevis


  • Ion Channels
  • Receptors, N-Methyl-D-Aspartate
  • DNA

Associated data

  • GENBANK/D12822
  • GENBANK/S39996
  • GENBANK/S40001
  • GENBANK/S40008
  • GENBANK/S40009
  • GENBANK/S40010
  • GENBANK/X65120
  • GENBANK/X72578
  • GENBANK/X72579
  • GENBANK/X72580