Fusion of the leucine zipper gene HLF to the E2A gene in human acute B-lineage leukemia

Science. 1992 Jul 24;257(5069):531-4. doi: 10.1126/science.1386162.

Abstract

A t(17;19) chromosomal translocation in early B-lineage acute leukemia was shown to result in chimeric transcripts that contain sequences from the E2A basic helix-loop-helix transcription factor gene on chromosome 19, fused to sequences from a previously unidentified gene (HLF) on chromosome 17 that encodes a hepatic leukemia factor. The chimeric protein consisted of the amino-terminal transactivation domain of E2A linked to the carboxyl-terminal basic region-leucine zipper domain of HLF. HLF was normally expressed in liver and kidney, but not in lymphoid cells, and was found to be closely related to the leucine zipper-containing transcription factors DBP (albumin D-box binding protein) and TEF (thyrotroph embryonic factor), which regulate developmental stage-specific gene expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Early Proteins
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Burkitt Lymphoma / genetics*
  • Cell Line
  • Chromosomes, Human, Pair 17*
  • Chromosomes, Human, Pair 19*
  • Cloning, Molecular
  • Humans
  • Leucine Zippers / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Oligodeoxyribonucleotides
  • Oncogene Proteins, Viral / genetics*
  • Polymerase Chain Reaction / methods
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / genetics*
  • Translocation, Genetic*

Substances

  • Adenovirus Early Proteins
  • Oligodeoxyribonucleotides
  • Oncogene Proteins, Viral
  • RNA, Neoplasm
  • Transcription Factors

Associated data

  • GENBANK/M95585
  • GENBANK/M95586