Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs

Plant Mol Biol. 1992 Aug;19(5):781-92. doi: 10.1007/BF00027074.

Abstract

We have cloned and sequenced three different cDNAs from sunflower seed-stored mRNA. Sequence similarities and response to heat-shock identified one of the cDNAs as a low-molecular-weight heat-shock protein (lmw-HSP). The other two clones showed significant sequence similarity to the cotton and carrot late-embryogenesis-abundant (Lea) proteins D-113 and Emb-1, respectively. The three cDNAs showed similar expression patterns during zygotic embryo development, as well as in vegetative tissues of 3-day-old seedlings in response to stress. Maximal accumulation of all three mRNAs was detected in dry seeds and during embryo mid-maturation stage, in the absence of exogenous stress. In seedlings, mRNAs accumulated to lower levels in response to osmotic stress and exogenous abscisic acid (ABA) treatments. A differential time course of response to osmotic stress was observed: lmw-HSP mRNA accumulation was induced earlier than that of Lea mRNAs. The coordinate accumulation of Lea and lmw-HSP transcripts during embryo development and in response to stress and ABA suggests the existence of common regulatory elements for Lea and lmw-HSP genes, and supports the notion that HSPs might have alternative functions in the plant cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics*
  • Helianthus / genetics*
  • Helianthus / growth & development
  • Molecular Sequence Data
  • Osmotic Pressure
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • RNA, Messenger / genetics
  • Seeds / genetics*

Substances

  • Heat-Shock Proteins
  • Plant Proteins
  • RNA, Messenger
  • late embryogenesis abundant protein, plant
  • Abscisic Acid

Associated data

  • GENBANK/X59699
  • GENBANK/X59700
  • GENBANK/X59701