Analysis of immature (CD4-CD8-) thymic subsets in T-cell receptor alpha beta transgenic mice

Dev Immunol. 1992;2(2):85-94. doi: 10.1155/1992/45150.


Introduction of a transgenic alpha beta TCR (V alpha 2, V beta 8.1) specific for lymphocytic choriomeningitis virus (LCMV), in the context of H-2Db into the genome of C57BL/6 mice, has many effects on the development and selection of T cells in both the thymus and the periphery. These mice produce increased numbers of CD4-8+ mature T cells, all of which express the transgenic TCR, and small numbers of CD4+8- cells using endogenous TCRs are also produced. This study follows the intrathymic development of T cells in these TCR alpha beta transgenic mice, in particular the earliest CD4-8- stages. As expected, the transgenic TCR is expressed on the cell surface at an earlier developmental stage than endogenous TCRs in nontransgenic littermate controls. Of the three major subsets expressing the heat-stable antigen (HSA), only the most mature, the CD25-CD44- expresses the transgenic TCR, and the earlier CD25-CD44+ and CD25+CD44- do not. Furthermore, in contrast to other TCR alpha beta transgenic lines, TCR gamma delta lineage cells appear to develop normally.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • CD4 Antigens / analysis*
  • CD8 Antigens / analysis*
  • Cell Differentiation
  • Gene Expression Regulation
  • Mice
  • Mice, Transgenic / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • T-Lymphocyte Subsets / immunology*
  • Thymus Gland / growth & development*
  • Thymus Gland / immunology


  • Antibodies, Monoclonal
  • CD4 Antigens
  • CD8 Antigens
  • Receptors, Antigen, T-Cell, alpha-beta