The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes

J Exp Med. 1962 Mar 1;115(3):453-66. doi: 10.1084/jem.115.3.453.


An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. Antibody-antigen mixtures when incubated (37 degrees C) in medium containing fresh (i.e. non-inactivated) normal rabbit serum exert a strong chemotactic effect on rabbit polymorphonuclear leucocytes. Results are described which indicate that, when antibody-antigen complexes are incubated (37 degrees C) in fresh serum, a heat-stable (56 degrees C) substance (or substances) is produced which acts directly as a chemotactic stimulus on the polymorphs. This heat-stable chemotactic substance is not produced when antibody-antigen complexes are incubated in serum which has been heated at 56 degrees C for 30 minutes.

MeSH terms

  • Animals
  • Antibodies*
  • Antigen-Antibody Complex*
  • Antigens*
  • Leukocytes / chemistry*
  • Neutrophils*
  • Rabbits


  • Antibodies
  • Antigen-Antibody Complex
  • Antigens