Nuclear internalisation and DNA binding activities of interleukin-1, interleukin-1 receptor and interleukin-1/receptor complexes

Biochem Biophys Res Commun. 1992 Sep 16;187(2):1166-71. doi: 10.1016/0006-291x(92)91319-l.

Abstract

This paper presents evidence to suggest that interleukin-1 alpha as a complex with its receptor is able to express DNA binding activity. Both the interleukin-1/receptor complex and the interleukin-1 receptor appear to be able to bind to DNA, however interleukin-1 on its own showed no binding activity. Interleukin-1 was found to be internalised into the nuclei of all cells examined (EL4, MEL, HL-60, K562, THP-1 and Jurkat cells). The data suggest the possible modulation of genes by interaction of interleukin-1/receptor complexes with DNA structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Nucleus / metabolism*
  • Chromatography
  • Collodion
  • DNA / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Interleukin-1 / metabolism*
  • Iodine Radioisotopes
  • Kinetics
  • Receptors, Immunologic / metabolism*
  • Receptors, Interleukin-1

Substances

  • Interleukin-1
  • Iodine Radioisotopes
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Collodion
  • DNA