cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta

DNA Cell Biol. 1992 Sep;11(7):511-22. doi: 10.1089/dna.1992.11.511.


Transforming growth factor-beta (TGF-beta) is capable of affecting the proliferation of many cell types. To identify novel genes whose protein products may mediate cellular responses to this factor, a cDNA library was made from mRNA isolated from a human lung adenocarcinoma cell line (A549) that had been treated for 3 days with TGF-beta. The library was screened by differential hybridization and a cDNA clone, beta ig-h3, was isolated. This gene was induced up to 20-fold in A549 cells after 2 days of treatment with TGF-beta 1. It was also induced in several other cell lines, including PC-3 and H2981. DNA sequence analysis of beta ig-h3 indicated that it encoded a novel protein, beta IG-H3, of 683 amino acids, which contained an amino-terminal secretory sequence and a carboxy-terminal Arg-Gly-Asp (RGD) sequence that can serve as a ligand recognition site for several integrins. beta IG-H3 also contained short amino acid regions homologous to similar regions in Drosophila fasciclin-I and four homologous internal domains, which can be folded into a potential bivalent structure and could act as a bridge between cells expressing the appropriate ligand. beta ig-h3 RNA was detected in several cell lines and tissues. COS cells transfected with plasmids encoding beta IG-H3 secreted a major 68-kD protein that was detected by immunoblotting using antipeptide antibodies. Since beta ig-h3 is induced in several cell lines whose proliferation is affected by TGF-beta 1, it may be involved in mediating some of the signals of this multifunctional growth modulator.

MeSH terms

  • Adenocarcinoma
  • Amino Acid Sequence
  • Base Sequence
  • Cell Differentiation / genetics
  • Cell Division / genetics
  • Cell Line
  • Cloning, Molecular
  • DNA
  • Extracellular Matrix Proteins*
  • Humans
  • Immunoblotting
  • Kinetics
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Proteins / genetics*
  • Proteins / physiology
  • Sequence Homology
  • Transcription, Genetic
  • Transfection
  • Transforming Growth Factor beta / pharmacology*
  • Tumor Cells, Cultured


  • Extracellular Matrix Proteins
  • Neoplasm Proteins
  • Proteins
  • Transforming Growth Factor beta
  • betaIG-H3 protein
  • DNA

Associated data

  • GENBANK/L08493
  • GENBANK/L08494
  • GENBANK/L08495
  • GENBANK/L08496
  • GENBANK/L08497
  • GENBANK/M77349
  • GENBANK/S38220
  • GENBANK/S38221
  • GENBANK/S72771
  • GENBANK/X65482