Analysis of prostatic tumor cultures using fluorescence in-situ hybridization (FISH)

Cancer Genet Cytogenet. 1992 Sep;62(2):180-5. doi: 10.1016/0165-4608(92)90259-b.


Analysis of ten primary prostatic tumor cultures using fluorescence in-situ hybridization (FISH) with pericentromeric probes for chromosomes 7, 8, 10, 16, 17, and 18 revealed aneusomies in nine of these specimens. Classical cytogenetics by G-banding indicated that only four of those same ten specimens had any (but not consistent) clonal abnormalities. This preliminary study suggests that aneusomy is a common event in early-stage prostatic tumors, and also supports the notion that multiple chromosomes are involved. In combination with routine cytogenetic analysis, FISH is thus likely to be a powerful tool in the evaluation of prostatic cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Aberrations*
  • Culture Techniques
  • Evaluation Studies as Topic
  • Fluorescence
  • Humans
  • In Situ Hybridization
  • Male
  • Prostatic Neoplasms / genetics*