The plastome-encoded zfpA gene of a moss contains procaryotic as well as eucaryotic promoter consensus sequences and its RNA abundance is modulated by cytokinin

Curr Genet. 1992 Oct;22(4):327-33. doi: 10.1007/BF00317930.


Plastid DNA of the moss Physcomitrella patens has been sequenced. An open reading frame (ORF 315) was identified downstream from rbcL, between trnR-CCG and psaI. This ORF shares homology with zfpA, a putative regulatory gene in Pisum sativum. The moss ORF is preceded by a Shine-Dalgarno sequence, two plastid promoter consensus sequences, and three TATA boxes. A specific probe detected three transcripts of low abundance in the wild-type moss and a cytokinin-sensitive chloroplast mutant. Steady state levels of zfpA transcripts were different in the two genotypes. In mutant protonemata treated with cytokinin, steady state levels of the largest transcript decreased significantly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Chloroplasts
  • Cloning, Molecular
  • Cytokinins / pharmacology*
  • DNA, Circular
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation
  • Genes, Plant*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Plants / genetics*
  • Promoter Regions, Genetic*
  • RNA, Ribosomal
  • Transcription, Genetic / drug effects*
  • Zinc Fingers


  • Cytokinins
  • DNA, Circular
  • DNA-Binding Proteins
  • RNA, Ribosomal

Associated data

  • GENBANK/S45007
  • GENBANK/S72771
  • GENBANK/X59787
  • GENBANK/X60211
  • GENBANK/X62119
  • GENBANK/X65478
  • GENBANK/X65479
  • GENBANK/X65480
  • GENBANK/X65481
  • GENBANK/X65482