Molecular characterization of a mouse Y chromosomal repetitive sequence that detects transcripts in the testis

Cytogenet Cell Genet. 1992;61(2):87-90. doi: 10.1159/000133375.

Abstract

145SC5 is a Y chromosomal repetitive sequence isolated from a BALB/c mouse and is unique in that it detects poly(A)-containing transcripts in the testis. We determined nucleotide sequences of 145SC5 and a related cDNA clone designated PC11 and compared their sequences to that of pYMT2/B, another related cDNA clone. 145SC5 and PC11 are almost identical (97%), while PC11 and pYMT2/B share 84% identity. In situ hybridization and Southern blot analysis with XXSxr male mice demonstrated that 145SC5-related sequences were distributed over the entire length of the Y chromosome including the short arm. Two types of Y chromosome are present in inbred mouse strains: the Mus musculus musculus type and M.m. domesticus type. Among 26 inbred strains surveyed, 145SC5 detected polymorphims only in the M.m. domesticus Y chromosome.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • DNA / genetics*
  • DNA / isolation & purification
  • Karyotyping
  • Liver / physiology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Testis / physiology*
  • Transcription, Genetic*
  • Y Chromosome*

Substances

  • DNA

Associated data

  • GENBANK/L08491
  • GENBANK/L08492
  • GENBANK/L08493
  • GENBANK/L08494
  • GENBANK/L08495
  • GENBANK/L08496
  • GENBANK/L08497
  • GENBANK/S46515
  • GENBANK/S72771
  • GENBANK/X65482