The PSI-E subunit of photosystem I binds ferredoxin:NADP+ oxidoreductase

FEBS Lett. 1992 Oct 19;311(2):169-73. doi: 10.1016/0014-5793(92)81391-x.

Abstract

A photosystem I complex containing the polypeptides PSI-A to PSI-L, light-harvesting complex I and ferredoxin:NADP+ oxidoreductase has been isolated from barley using the non-ionic detergent n-decyl-beta-D-maltopyranoside. The ratio between bound ferredoxin:NADP+ oxidoreductase and P700 is 0.4 +/- 0.2. The complex is highly active in catalyzing light-induced transfer of electrons from plastocyanin to NADP+ at rates of 280 +/- 150 and 1800 +/- 800 mumol NADPH/(mg chl.h), without and in the presence of saturating amounts of exogenously added ferredoxin:NADP+ oxidoreductase, respectively. Endogenously bound ferredoxin:NADP+ oxidoreductase interacts with the PSI-E subunit as demonstrated by cross-linking experiments using two different types of cross-linkers and identification of the products by Western blotting and the use of monospecific antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents
  • Electron Transport
  • Ethyldimethylaminopropyl Carbodiimide / pharmacology
  • Ferredoxin-NADP Reductase / metabolism*
  • Hordeum / chemistry
  • Light
  • Molecular Weight
  • Photosynthetic Reaction Center Complex Proteins / isolation & purification
  • Photosynthetic Reaction Center Complex Proteins / metabolism*
  • Photosystem I Protein Complex
  • Plastocyanin / metabolism
  • Succinimides / pharmacology

Substances

  • Cross-Linking Reagents
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem I Protein Complex
  • Succinimides
  • 3,3'-dithiobis(sulfosuccinimidyl propionate)
  • Plastocyanin
  • Ferredoxin-NADP Reductase
  • Ethyldimethylaminopropyl Carbodiimide