A study of blood cultures from 320 cases of neonatal sepsis showed 136 (42.5%) to be positive for bacterial growth; of these 82 (60.29%) isolates being gram negative bacilli. Citrobacter was the commonest gram negative bacilli isolated. Other commonly isolated gram negative organisms were Pseudomonas, Klebsiella, Salmonella typhimurium, Acinetobacter and Escherichia coli. Antibiotics susceptibility pattern revealed the isolates to be resistant to commonly used antibiotics.