Serotonin uptake by astrocytes in situ

Glia. 1992;6(2):154-8. doi: 10.1002/glia.440060210.


Co-localization of glial fibrillary acidic protein (GFAP) and radioactivity was examined after intraventricular injection of [3H]5-HT in adult rat brains. Radioactivity localized over GFAP-positive astrocytes was seen, especially when image-enhancing techniques were applied to the data. Also slices prepared from astrogliotic hippocampi of rats pretreated with kainic acid showed a twofold increased uptake of [3H]5-HT compared to control slices. This indicates that the uptake of [3H]5-HT seen in primary astrocyte cultures also occurs for astrocytes in situ. Also, as with astrocyte cultures, only some of the GFAP(+) astrocytes in situ showed localization of radioactivity, supporting the concept of intraregional heterogeneity of astrocyte functions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Astrocytes / metabolism*
  • Brain Chemistry / physiology
  • Gliosis / metabolism
  • Hippocampus / metabolism
  • Immunohistochemistry
  • In Vitro Techniques
  • Injections, Intraventricular
  • Kainic Acid / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin / metabolism*


  • Serotonin
  • Kainic Acid