Identification of acidic mucopolysaccharides by agarose gel electrophoresis

J Chromatogr. 1977 Jan 11:130:299-304. doi: 10.1016/s0021-9673(00)89809-x.


A micro-method for the identification of most acidic mucopolysaccharides by agarose gel electrophoresis with three different buffer systems is described. In barbital buffer the mucopolysaccharides are fractionated from each other as a function of their net charge, whereas in a diamine buffer the fractionation is probably achieved according to the degree to which they are bound to the diamine. A combination of barbital and diaminopropane buffers in two-dimensional electrophoresis for the identification of mucopolysaccharides is also described.

MeSH terms

  • Electrophoresis, Agar Gel
  • Glycosaminoglycans / analysis*
  • Heparin / analysis
  • Heparitin Sulfate / analysis
  • Methods


  • Glycosaminoglycans
  • Heparin
  • Heparitin Sulfate