SOME ELECTRICAL PROPERTIES OF A NUCLEAR MEMBRANE EXAMINED WITH A MICROELECTRODE

J Gen Physiol. 1963 Jul;46(6):1123-40. doi: 10.1085/jgp.46.6.1123.

Abstract

Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 ohm cm(2) and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally.

MeSH terms

  • Animals
  • Cell Nucleus*
  • Cytoplasm*
  • Drosophila*
  • Electrophysiology*
  • Ions*
  • Membrane Potentials*
  • Microelectrodes*
  • Nuclear Envelope*
  • Salivary Glands*

Substances

  • Ions