TRANSCRIPTION IN VIVO OF DNA FROM BACTERIOPHAGE SP8

Science. 1963 Oct 18;142(3590):387-9. doi: 10.1126/science.142.3590.387.

Abstract

The DNA of bacteriophage SP8, when denatured, yields two components differing in buoyant density in cesium chloride gradients and separable by chromatography on a column of methylated bovine serum albumin and kieselguhr. The denser of the two strands (H) contains more pyrimidines and fewer purines than the lighter (L) strand. Only the H strand forms hybrids with the RNA synthesized by the infected host. The L strand is capable of annealing with complementary RNA synthesized in vitro with it as primer in reactions catalyzed by RNA polymerase. During the vegetative development of phage, host-specific messenger RNA is also synthesized.

MeSH terms

  • Bacillus subtilis*
  • Bacteriophages*
  • DNA*
  • DNA, Viral*
  • DNA-Directed RNA Polymerases*
  • RNA*
  • RNA, Messenger*
  • RNA, Viral*
  • Research*
  • Transcription, Genetic*

Substances

  • DNA, Viral
  • RNA, Messenger
  • RNA, Viral
  • RNA
  • DNA
  • DNA-Directed RNA Polymerases