Continuum of overlapping clones spanning the entire human chromosome 21q

Nature. 1992 Oct 1;359(6394):380-7. doi: 10.1038/359380a0.


A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping / methods*
  • Chromosomes, Fungal
  • Chromosomes, Human, Pair 21*
  • Cloning, Molecular
  • Gene Library
  • Genetic Markers
  • Genome, Human*
  • Humans
  • Polymerase Chain Reaction
  • Sequence Tagged Sites


  • Genetic Markers