THYMINE DIMERS AND INHIBITION OF DNA SYNTHESIS BY ULTRAVIOLET IRRADIATION OF CELLS

Science. 1963 Dec 13;142(3598):1464-6. doi: 10.1126/science.142.3598.1464.

Abstract

Measurements of the inhibition of DNA synthesis in bacteria and the formation of thymine dimers by ultraviolet irradiation of the cells indicate that one thymine dimer per 350-IA strand of DNA acts as a block to further synthesis. In a sensitive strain of Escherichia coli the blocks are permanent. In a resistant strain the blocks are only temporary but recovery of synthesis is not the result of splitting dimers.

MeSH terms

  • DNA Replication*
  • DNA, Bacterial*
  • Escherichia coli*
  • Proteins / metabolism*
  • Pyrimidine Dimers*
  • Radiation Effects*
  • Thymine*
  • Ultraviolet Rays*

Substances

  • DNA, Bacterial
  • Proteins
  • Pyrimidine Dimers
  • Thymine