Use of a recombinant Dictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

Parasitol Res. 1992;78(4):298-302. doi: 10.1007/BF00937087.

Abstract

A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected with Dictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of greater than 99% for both antigen preparations and a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and greater than 99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / blood*
  • Antigens, Helminth*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Dictyocaulus / immunology*
  • Dictyocaulus Infections / diagnosis*
  • Enzyme-Linked Immunosorbent Assay
  • Predictive Value of Tests
  • Recombinant Proteins
  • Regression Analysis
  • Sensitivity and Specificity

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Recombinant Proteins