Structural principles for the propeller assembly of beta-sheets: the preference for seven-fold symmetry

Proteins. 1992 Oct;14(2):191-201. doi: 10.1002/prot.340140206.

Abstract

Twisted beta-sheets, packed face to face, may be arranged in circular formation like blades of a propeller or turbine. This beta-propeller fold has been found in three proteins: that in neuraminidase consists of six beta-sheets while those in methylamine dehydrogenase and galactose oxidase are composed of seven beta-sheets. A model for multisheet packing in the beta-propeller fold is proposed. This model gives both geometrical parameters of the beta-propellers composed of different numbers of sheets and patterns of residue packing at their sheet-to-sheet interfaces. All the known beta-propeller structures have been analyzed, and the observed geometries and residue packing are found to be in good agreement with those predicted by models. It is shown that unusual seven-fold symmetry is preferable to six- or eight-fold symmetry for propeller-like multi-sheet assembly. According to the model, a six-beta-sheet propeller has to have predominantly small residues in the beta-strands closed to its six-fold axis, but no strong sequence constraints are necessary for a seven-fold beta-propeller.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Galactose Oxidase / chemistry
  • Models, Molecular
  • Neuraminidase / chemistry
  • Oxidoreductases Acting on CH-NH Group Donors / chemistry
  • Protein Structure, Secondary*

Substances

  • Galactose Oxidase
  • methylamine dehydrogenase
  • Oxidoreductases Acting on CH-NH Group Donors
  • Neuraminidase