Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9809-13. doi: 10.1073/pnas.89.20.9809.


cDNA encoding a hormone- and guanine nucleotide-stimulated adenylyl cyclase [ATP pyrophosphate-lyase (cyclizing), EC] (type 6) from rat liver and kidney has been cloned and expressed. This enzyme is stimulated by forskolin, guanosine 5'-[gamma-thio]triphosphate, and isoproterenol plus GTP but is not stimulated by beta gamma subunits of guanine nucleotide-binding proteins. A second form (type 5), which is 75% similar to type 6, has also been cloned. Both types 5 and 6 cDNAs have multiple messages. PCR-based detection of the mRNA for the type 5 and 6 enzymes indicates that both are widely distributed. Homology analyses indicate at least four distinct subfamilies of guanine nucleotide stimulatory protein-regulated adenylyl cyclases. Types 5 and 6 enzymes define one distinct subfamily of mammalian adenylyl cyclases. Diversity of one guanine nucleotide-binding protein-regulated effector may allow different modes of regulation of cell-surface signal transmission.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / classification
  • Adenylyl Cyclases / genetics*
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Colforsin / pharmacology
  • DNA / genetics
  • Gene Expression / drug effects
  • Isoproterenol / pharmacology
  • Kidney / enzymology
  • Liver / enzymology
  • Membrane Proteins / classification
  • Membrane Proteins / ultrastructure
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • Membrane Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Colforsin
  • DNA
  • Adenylyl Cyclases
  • Isoproterenol

Associated data

  • GENBANK/M96159
  • GENBANK/M96160