The Clonogen Content of Murine Intestinal Crypts: Dependence on Radiation Dose Used in Its Determination

Radiat Res. 1992 Oct;132(1):115-9.


The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 +/- 0.8 cells using two equal doses each less than 9 Gy and 38 +/- 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colony-Forming Units Assay / methods*
  • Dose-Response Relationship, Radiation
  • Intestine, Small / cytology
  • Intestine, Small / radiation effects*
  • Male
  • Mice
  • Stem Cells / radiation effects*