Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor

Biochem Biophys Res Commun. 1992 Sep 30;187(3):1579-86. doi: 10.1016/0006-291x(92)90483-2.


Vascular endothelial cell growth factor (VEGF), also known as vascular permeability factor, is an endothelial cell mitogen which stimulates angiogenesis. Here we report that a previously identified receptor tyrosine kinase gene, KDR, encodes a receptor for VEGF. Expression of KDR in CMT-3 (cells which do not contain receptors for VEGF) allows for saturable 125I-VEGF binding with high affinity (KD = 75 pM). Affinity cross-linking of 125I-VEGF to KDR-transfected CMT-3 cells results in specific labeling of two proteins of M(r) = 195 and 235 kDa. The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, flt, in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Protein-Tyrosine Kinases / genetics*
  • Receptors, Mitogen / genetics*
  • Receptors, Vascular Endothelial Growth Factor
  • Sequence Alignment


  • Oligodeoxyribonucleotides
  • Receptors, Mitogen
  • Protein-Tyrosine Kinases
  • Receptors, Vascular Endothelial Growth Factor

Associated data

  • GENBANK/D10475
  • GENBANK/D10916
  • GENBANK/D10917
  • GENBANK/D10918
  • GENBANK/D10919
  • GENBANK/D10920
  • GENBANK/S41679
  • GENBANK/S41680
  • GENBANK/S41731
  • GENBANK/X61656