Direct evidence for protein kinase C involvement in insulin-stimulated hexose uptake

Biochem Biophys Res Commun. 1992 Oct 15;188(1):142-8. doi: 10.1016/0006-291x(92)92361-z.


Insulin has been reported to translocate protein kinase C (PKC) in rat adipocytes, and activation of PKC by phorbol esters is known to increase hexose uptake in these cells (1.2). To test the hypothesis that PKC may participate in insulin-stimulated hexose uptake, adipocytes were partially depleted of protein kinase C by overnight phorbol ester treatment, thereby impairing insulin effects on hexose uptake. Purified PKC was then introduced into these PKC-depleted adipocytes by electropermeabilization, and this fully restored insulin-stimulated hexose uptake. These findings provide direct evidence that PKC is required for insulin-stimulated hexose uptake.

MeSH terms

  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Animals
  • Biological Transport / drug effects
  • Cells, Cultured
  • Deoxyglucose / metabolism*
  • Electric Stimulation
  • Glycerol / metabolism
  • Male
  • Monosaccharide Transport Proteins / metabolism*
  • Protein Kinase C / metabolism*
  • Rats
  • Tetradecanoylphorbol Acetate / pharmacology


  • Monosaccharide Transport Proteins
  • Deoxyglucose
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate
  • Glycerol