Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican

Biochem Biophys Res Commun. 1992 Oct 15;188(1):395-401. doi: 10.1016/0006-291x(92)92398-h.


We have obtained the complete coding sequence of a highly conserved heparan sulfate proteoglycan which we previously characterized biochemically after isolation from rat brain. An open reading frame of 558 amino acids encodes a protein with a molecular mass of 62 kDa containing three peptide sequences present in the isolated proteoglycan. The total sequence obtained is 3.5 kb long, including 1.6 kb of 3'-untranslated sequence and 0.2 kb of 5'-untranslated sequence. The deduced amino acid sequence and the 3'- and 5'-untranslated sequences have 89% and 66-80% identity, respectively, with those of a phosphatidylinositol-anchored human lung fibroblast heparan sulfate proteoglycan (glypican) for which mRNA is detectable in a large number of human cell lines. Our data therefore demonstrate that this major heparan sulfate proteoglycan of brain is the rat form of glypican.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / physiology*
  • Cloning, Molecular / methods
  • DNA / genetics
  • Gene Library
  • Heparan Sulfate Proteoglycans
  • Heparitin Sulfate / genetics*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Open Reading Frames
  • Peptide Fragments / chemistry
  • Polymerase Chain Reaction
  • Proteoglycans / genetics*
  • Rats


  • Heparan Sulfate Proteoglycans
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Peptide Fragments
  • Proteoglycans
  • DNA
  • Heparitin Sulfate

Associated data

  • GENBANK/D10916
  • GENBANK/D10917
  • GENBANK/D10918
  • GENBANK/D10919
  • GENBANK/D10920
  • GENBANK/L02896
  • GENBANK/S41680
  • GENBANK/S41731
  • GENBANK/S46789
  • GENBANK/S46790