Oxidized HDL are much less cytotoxic to lymphoblastoid cells than oxidized LDL

Biochim Biophys Acta. 1992 Oct 30;1128(2-3):163-6. doi: 10.1016/0005-2760(92)90302-c.


The possible effect of oxidized HDL was investigated on lymphoblastoid cells, in comparison to the cytotoxic effect of oxidized LDL. Oxidation of HDL was promoted by UV-C irradiation, or by copper ion (5 microM) or the combination of the two treatments. HDL extensively treated by UV-C for 20 h did not exhibit any cytotoxic effect on cultured lymphoblastoid cells even at a concentration of 500 micrograms apolipoprotein A-I/ml. In contrast to UV-treated (2 h) LDL, which were highly cytotoxic (already at a concentration of 100 micrograms apolipoprotein B/ml), HDL treated by copper or copper + UV were oxidized, as shown by TBARS formation and PUFA content decrease, but were slightly cytotoxic.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arteriosclerosis / etiology
  • Cell Line / drug effects
  • Copper
  • Copper Sulfate
  • Fatty Acids, Unsaturated / analysis
  • Humans
  • L-Lactate Dehydrogenase / analysis
  • Lipoproteins, HDL / chemistry
  • Lipoproteins, HDL / toxicity*
  • Lipoproteins, LDL / chemistry
  • Lipoproteins, LDL / toxicity*
  • Oxidation-Reduction
  • Tetrazolium Salts
  • Thiazoles
  • Thiobarbituric Acid Reactive Substances / analysis
  • Trypan Blue
  • Ultraviolet Rays


  • Fatty Acids, Unsaturated
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Tetrazolium Salts
  • Thiazoles
  • Thiobarbituric Acid Reactive Substances
  • Copper
  • L-Lactate Dehydrogenase
  • thiazolyl blue
  • Trypan Blue
  • Copper Sulfate