The complete cDNA sequence for dihydrolipoyl transacylase (E2) of human branched-chain alpha-keto acid dehydrogenase complex

Biochim Biophys Acta. 1992 Oct 20;1132(3):319-21. doi: 10.1016/0167-4781(92)90169-z.

Abstract

We have determined the complete nucleotide sequence for the cDNA encoding human dihydrolipoyl transacylase (E2) using the rapid amplification of cDNA ends (RACE) procedure. The full-length E2 cDNA is 3535 nucleotides in length. The coding region spans 1446 bp and the 3'-noncoding region spans 2074 bp. The latter contains three Alu repetitive sequences and two transcription termination sites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Acyltransferases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • Humans
  • Ketone Oxidoreductases / genetics*
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*

Substances

  • Multienzyme Complexes
  • DNA
  • Ketone Oxidoreductases
  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Acyltransferases
  • dihydrolipoamide acyltransferase

Associated data

  • GENBANK/L07316
  • GENBANK/M91429
  • GENBANK/M91430
  • GENBANK/M93297
  • GENBANK/M93298
  • GENBANK/M93299
  • GENBANK/M93300
  • GENBANK/M93301
  • GENBANK/M94056
  • GENBANK/X66785