Different distributions of dystrophin and related proteins at nerve-muscle junctions

Neuroreport. 1992 Oct;3(10):857-60. doi: 10.1097/00001756-199210000-00009.


The distributions of dystrophin, 'dystrophin-related protein' (DRP) and beta-spectrin were compared with that of acetylcholine receptors (AChRs) at rat nerve-muscle junctions (NMJs) using immunofluorescence techniques. In sections, monoclonal antibodies (MAbs) to dystrophin and beta-spectrin labelled the entire sarcolemma but were concentrated at the NMJs while those to DRP labelled only NMJs. In permeabilized muscle fibres, DRP was precisely co-localized with the AChRs, whereas the zone of high density labelling of dystrophin and beta-spectrin extended 0.3-0.4 microns beyond the AChRs. Within the NMJ, the labelling of DRP appeared as a series of interconnecting lines similar to that of AChRs. However, labelling of dystrophin and beta-spectrin was consistently more punctate. These data suggest DRP is more closely associated with AChRs than are dystrophin or beta-spectrin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Cytoskeleton / metabolism
  • Dystrophin / metabolism*
  • Female
  • Immunohistochemistry
  • Neuromuscular Junction / metabolism*
  • Rats
  • Rats, Wistar
  • Receptors, Cholinergic / metabolism


  • Antibodies, Monoclonal
  • Dystrophin
  • Receptors, Cholinergic