Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins

Cell. 1992 Oct 16;71(2):323-34. doi: 10.1016/0092-8674(92)90360-o.


Murine D type cyclins associate with a catalytic subunit (p34PSK-J3) with properties distinct from known cyclin-dependent kinases (cdks). Mouse p34PSK-J3 shows less than 50% amino acid identity to p34cdc2, p33cdk2, and p36cdk3, lacks a PSTAIRE motif, and does not bind to p13suc1. Cyclin D1-p34PSK-J3 complexes accumulate in macrophages during G1 and decline in S phase, whereas complexes involving cyclins D2 and D3 form in proliferating T cells. Although histone H1 kinase activity is not detected in cyclin D or PSK-J3 immunoprecipitates, cyclin D-p34PSK-J3 complexes assembled in vitro stably bind and phosphorylate the retinoblastoma gene product (pRb) and an Rb-like protein (p107) but do not interact with pRb mutants that are functionally inactive. Thus, p34PSK-J3 is a cyclin D-regulated catalytic subunit that acts as an Rb (but not H1) kinase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CDC2 Protein Kinase
  • Cells, Cultured
  • Cyclin D1
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinases*
  • Cyclins / chemistry*
  • G1 Phase
  • Humans
  • Insecta
  • Macrophages
  • Mice
  • Molecular Sequence Data
  • Oncogene Proteins / chemistry*
  • Protein Kinases / chemistry*
  • Protein Kinases / genetics
  • Protein Kinases / isolation & purification
  • Proto-Oncogene Proteins*
  • Sequence Homology, Amino Acid


  • Cyclins
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Cyclin D1
  • Protein Kinases
  • CDC2 Protein Kinase
  • CDK4 protein, human
  • Cdk4 protein, mouse
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/L01640
  • GENBANK/M86183
  • GENBANK/M88182