A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia

Cell. 1992 Nov 13;71(4):587-97. doi: 10.1016/0092-8674(92)90593-2.


Cell cycle checkpoints can enhance cell survival and limit mutagenic events following DNA damage. Primary murine fibroblasts became deficient in a G1 checkpoint activated by ionizing radiation (IR) when both wild-type p53 alleles were disrupted. In addition, cells from patients with the radiosensitive, cancer-prone disease ataxia-telangiectasia (AT) lacked the IR-induced increase in p53 protein levels seen in normal cells. Finally, IR induction of the human GADD45 gene, an induction that is also defective in AT cells, was dependent on wild-type p53 function. Wild-type but not mutant p53 bound strongly to a conserved element in the GADD45 gene, and a p53-containing nuclear factor, which bound this element, was detected in extracts from irradiated cells. Thus, we identified three participants (AT gene(s), p53, and GADD45) in a signal transduction pathway that controls cell cycle arrest following DNA damage; abnormalities in this pathway probably contribute to tumor development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Ataxia Telangiectasia / genetics*
  • Ataxia Telangiectasia / pathology
  • Base Sequence
  • Cell Cycle / genetics
  • Cell Cycle / radiation effects*
  • Cell Line
  • Cloning, Molecular
  • DNA Damage / genetics*
  • DNA Repair / genetics
  • Gene Expression Regulation / radiation effects*
  • Genes, p53 / genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutagenesis / genetics

Associated data

  • GENBANK/M17851