Functional analysis of alternatively spliced transcripts of the human histidine decarboxylase gene and its expression in human tissues and basophilic leukemia cells

Eur J Biochem. 1992 Oct 15;209(2):533-9. doi: 10.1111/j.1432-1033.1992.tb17317.x.


L-Histidine decarboxylase (HisDC) is the enzyme catalyzing the formation of histamine from L-histidine. HisDC activity is expressed specifically in mast cells/basophils, endocrine cells in stomach, and histaminergic neurons in brain. As a first step in the analysis of the regulation of HisDC gene expression, we have cloned the cDNA coding for HisDC from a cDNA library of a human basophilic leukemia cell line, KU-812-F. We identified two types of HisDC cDNA, representing the 2.4-kb and 3.4-kb HisDC mRNA constitutively expressed in these cells. Sequence analysis of these cDNA revealed that the 3.4-kb mRNA contains the insert sequence of 824 bases and suggests that both 2.4-kb and 3.4-kb mRNA may represent the alternatively spliced transcripts of the HisDC gene. Using expression plasmids containing a cDNA for each HisDC mRNA, we analyzed the function of possible HisDC isoforms. We show that only the 2.4-kb mRNA encodes functional HisDC and is expressed in human brain and lung. However, we were unable to detect the 3.4-kb mRNA in these tissues. Thus, the 3.4-kb mRNA may be generated by KU-812-F cell-specific splicing of the HisDC gene transcripts. Furthermore, we demonstrated the increase in the level of 2.4-kb HisDC mRNA and HisDC activity in KU-812-F cells following treatment with phorbol 12-myristate 13-acetate.

Publication types

  • Comparative Study

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gene Expression
  • Gene Library
  • Histidine Decarboxylase / biosynthesis*
  • Histidine Decarboxylase / genetics*
  • Humans
  • Leukemia, Basophilic, Acute / enzymology*
  • Leukemia, Basophilic, Acute / genetics
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / genetics
  • Rats
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Histidine Decarboxylase

Associated data

  • GENBANK/D10088
  • GENBANK/M85175
  • GENBANK/M85176
  • GENBANK/M85177
  • GENBANK/S42403
  • GENBANK/S42404
  • GENBANK/X65179
  • GENBANK/X65180
  • GENBANK/X65181
  • GENBANK/X70868