Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation

Eur J Biochem. 1992 Nov 1;209(3):951-9. doi: 10.1111/j.1432-1033.1992.tb17368.x.


Trehalose-6-phosphate synthase is the key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells of yeast. This enzyme was purified from a strain of Saccharomyces cerevisiae lacking vacuolar proteases. It was found to be a multimeric protein of 630 kDa. Monoclonal antibodies were raised against its smallest subunit (56 kDa) and used for screening a yeast cDNA library. This yielded an immunopositive cDNA clone of 1.7 kb, containing an open reading frame of 1485 base pairs. Its sequence, called TPS1 (for trehalose-6-phosphate synthase), was represented by a single gene in the yeast genome and was found to be almost identical with the recently sequenced CIF1, a gene important for carbon catabolite inactivation, believed to be allelic with FDP1. A mutant obtained by disruption of TPS1 had a very low activity of trehalose-6-phosphate synthase, indicating that TPS1 is an important component of the enzyme. The mutant also showed a growth defect when transferred from glycerol to glucose, a phenotype similar to that of the cif1 and fdp1 mutants deficient in carbon catabolite inactivation. Thus, the smallest subunit of the biosynthetic enzyme trehalose-6-phosphate synthase appears to have, in addition, a central regulatory role in the carbohydrate metabolism of yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Carbon / metabolism*
  • Cloning, Molecular
  • DNA, Fungal
  • Electrophoresis, Polyacrylamide Gel
  • Glucosyltransferases / genetics*
  • Glucosyltransferases / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics


  • DNA, Fungal
  • Carbon
  • Glucosyltransferases
  • trehalose-6-phosphate synthase

Associated data

  • GENBANK/M85175
  • GENBANK/M85176
  • GENBANK/M85177
  • GENBANK/S42404
  • GENBANK/X65179
  • GENBANK/X65180
  • GENBANK/X65181
  • GENBANK/X68214
  • GENBANK/Z12621
  • GENBANK/Z12622