A number of apparently unrelated factors are known to have a profound effect on the metabolism of cultured mammalian cells; and some of these may be operative as metabolic controls in the whole animal as well. The more complete exploration of (i) homotypic and heterotypic cellular interactions, (ii) the spontaneous transformations sometimes observed in cultured cells, (iii) the mode of action of cytotoxic agents, (iv) the multiple metabolic effects of viral infection, and (v) the conditions necessary for the maintenance of specialized function in cultured cells, can be expected to throw light on the basic mechanisms underlying such complex processes as differentiation, senescence, and cancer.