Cloning and sequencing of a plasmid-mediated erythromycin resistance determinant from Staphylococcus xylosus

FEMS Microbiol Lett. 1992 Oct 1;76(1-2):141-7. doi: 10.1016/0378-1097(92)90377-z.


A 2.3-kb DNA fragment cloned from plasmid pCH200, the largest (52 kb) of four plasmids detected in Staphylococcus xylosus, was found to confer resistance to 14-membered ring macrolides in Bacillus subtilis and Staphylococcus aureus. DNA-sequence analysis of the fragment revealed the presence of an open-reading frame, the deduced product of which was identical to one of the two ATP-binding domains encoded by the macrolide/streptogramin-B-resistance gene msrA of Staphylococcus epidermidis. The observation that a polypeptide homologous to the C-terminus of MsrA is capable of mediating erythromycin resistance in the absence of the N-terminal region is of significance both to the evolution and functional activity of members of the ATP-binding transport super-gene family.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Drug Resistance, Microbial / genetics*
  • Erythromycin / pharmacology
  • Genes, Bacterial
  • Molecular Sequence Data
  • Plasmids
  • Staphylococcus / drug effects
  • Staphylococcus / genetics*


  • DNA, Bacterial
  • Erythromycin

Associated data

  • GENBANK/M81802