Detection of a polymorphism within the pepsinogen C gene with PCR: construction of a linkage map around PGC from 6p11-6p21.3

Genomics. 1992 Oct;14(2):398-402. doi: 10.1016/s0888-7543(05)80232-0.


An insertion/deletion polymorphism between exons 7 and 8 of the pepsinogen C gene (PGC), previously detectable with Southern analysis, was formatted for detection with PCR. Alleles were rapidly typed by UV irradiation of ethidium bromide-stained agarose gels. Whereas Southern analysis revealed two alleles, the smaller fragments generated with PCR allowed the resolution of three alleles that were previously scored as a single allele and increased the heterozygosity of the system from 0.20 to 0.53. After a set of reference families was genotyped with the PCR-based polymorphism, a linkage map around the PGC gene on chromosome 6 was constructed. This included the HLA cluster and the highly informative D6S223 locus. PGC lies 22 cM proximal to HLA-DPB and between D6S5 and D6S4 at distances of 4.5 and 13.1 cM, respectively.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 6*
  • DNA, Single-Stranded
  • Female
  • Genetic Linkage
  • Heterozygote
  • Humans
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Pepsinogens / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*


  • DNA, Single-Stranded
  • Pepsinogens

Associated data

  • GENBANK/L14832
  • GENBANK/M93323
  • GENBANK/M93325
  • GENBANK/M94357
  • GENBANK/M94358
  • GENBANK/M94359
  • GENBANK/M94360
  • GENBANK/M94361
  • GENBANK/M96835
  • GENBANK/X15965