The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments

J Gen Virol. 1992 Nov;73 ( Pt 11):2795-804. doi: 10.1099/0022-1317-73-11-2795.

Abstract

The complete sequence of the tomato spotted wilt virus (TSWV) M RNA segment has been determined. The RNA is 4821 nucleotides long and has an ambisense coding strategy similar to that of the S RNA segment. The M RNA segment contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted size of 33.6K, and one in the viral complementary sense which encodes the precursor to the G1 and G2 glycoproteins, with a predicted size of 127.4K. Both ORFs are expressed via the synthesis of subgenomic mRNAs that possibly terminate at a stable hairpin structure, located in the intergenic region. The precursor for the glycoproteins contains a sequence motif (RGD) which is characteristic of cellular attachment domains. Significant sequence homology was found between the G1 glycoproteins of members of the genus Bunyavirus and a corresponding region in the glycoprotein precursor of TSWV, indicating a close evolutionary relationship between these viruses. With the elucidation of the M RNA sequence, the complete nucleotide sequence of TSWV has been determined. TSWV represents the first member of the Bunyaviridae shown to contain two ambisense RNA segments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Bunyamwera virus / genetics
  • Bunyaviridae / genetics*
  • Cloning, Molecular
  • Genome, Viral
  • Molecular Sequence Data
  • Open Reading Frames
  • Plant Diseases / microbiology
  • Plant Viruses / genetics*
  • Plants / microbiology
  • Plants, Toxic
  • Protein Biosynthesis
  • Protein Structure, Secondary
  • RNA, Viral / genetics*
  • Sequence Homology, Nucleic Acid
  • Tobacco / microbiology
  • Transcription, Genetic*
  • Viral Matrix Proteins / genetics*

Substances

  • RNA, Viral
  • Viral Matrix Proteins