Abstract
Processing of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5 could be mediated by the catalytically active NS3 protease domain and NS2B in trans at the dibasic sites NS3-NS4A and NS4B-NS5. Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Dengue Virus / genetics
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Dengue Virus / metabolism*
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Organelles / metabolism
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Organelles / ultrastructure
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Plasmids
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Polymerase Chain Reaction / methods
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Protein Precursors / analysis
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Protein Precursors / metabolism*
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Protein Processing, Post-Translational*
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Viral Nonstructural Proteins / biosynthesis
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism*
Substances
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Oligodeoxyribonucleotides
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Protein Precursors
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Viral Nonstructural Proteins