Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies

Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10159-63. doi: 10.1073/pnas.89.21.10159.

Abstract

Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cell Cycle / drug effects*
  • Cell Division / drug effects*
  • Cricetinae
  • DNA / genetics
  • Gene Amplification
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Kinetics
  • Phorbol 12,13-Dibutyrate / metabolism
  • Protein Binding
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • Rats
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Transfection

Substances

  • Isoenzymes
  • Phorbol 12,13-Dibutyrate
  • DNA
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate