The role of BCG/PPD-activated macrophages in resistance against systemic candidiasis in mice

Scand J Immunol. 1992 Nov;36(5):713-9. doi: 10.1111/j.1365-3083.1992.tb03132.x.

Abstract

The main conclusions of this study are that BCG/PPD-activated macrophages, in contrast to macrophages from control mice, exhibit an increased PMA-induced production of H2O2, kill about one-third of the phagocytosed Candida albicans, and cause more than 50% inhibition of the intracellular formation of germ tubes by C. albicans. Peritoneal macrophages from mice that were colonized post-natally with C. albicans do not show increased production of H2O2 upon stimulation with PMA and the intracellular outgrowth of germ tubes is inhibited to only a limited degree. These macrophages are capable of killing about 20% of the ingested C. albicans. In vivo, the number of Candida in the kidney, spleen and liver after intravenous injection of Candida albicans is significantly lower in BCG-treated mice than in control mice. Post-natal colonization with C. albicans has only a limited effect on the outgrowth of intravenously injected C. albicans in the spleen and liver but does not influence growth in the kidney. These results indicate that acquired immunity against a systemic Candida infection involves both oxidative and non-oxidative mechanisms of intracellular killing and that these mechanisms may have different effects on the yeast and hyphal forms of C. albicans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Candidiasis / immunology*
  • Hydrogen Peroxide / metabolism
  • Macrophage Activation*
  • Macrophages / immunology*
  • Male
  • Mice
  • Mycobacterium bovis / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tuberculin / immunology*

Substances

  • Tuberculin
  • Hydrogen Peroxide
  • Tetradecanoylphorbol Acetate