An anatomic, histologic, and cytogenetic study was carried out on a young single-born ram of 'Bergamasca' breed. At an anatomical level the subject, with anomalous presence of horns adherent to the head, presented hypoplastic testicles descended in two distinct scrotal sacs, hypospadias and small penioid body. The histopathologic analysis showed atrophy of the testicular parenchyma while at a cytogenetic level, sexual chromosome mosaicism was found with XX and XY cells, both at lymphocytic (73%-54, XX; 27%-54, XY) and fibroblastic level. Therefore this finding cannot be explained simply referring to freemartinism.