Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

Anticancer Res. 1992 Sep-Oct;12(5):1377-82.

Abstract

Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Cisplatin / pharmacology
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / surgery
  • Coloring Agents
  • Doxorubicin / pharmacology
  • Drug Screening Assays, Antitumor
  • Esophageal Neoplasms / pathology
  • Esophageal Neoplasms / surgery
  • Fluorouracil / pharmacology
  • Gastrointestinal Neoplasms / pathology*
  • Gastrointestinal Neoplasms / surgery
  • Humans
  • Leiomyosarcoma / pathology
  • Leiomyosarcoma / surgery
  • Liver Neoplasms / pathology
  • Liver Neoplasms / surgery
  • Mitomycin / pharmacology
  • Rectal Neoplasms / pathology
  • Rectal Neoplasms / surgery
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / surgery
  • Tetrazolium Salts*
  • Thiazoles*
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Coloring Agents
  • Tetrazolium Salts
  • Thiazoles
  • Mitomycin
  • Doxorubicin
  • thiazolyl blue
  • Cisplatin
  • Fluorouracil