Abstract
Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Cell Division / drug effects
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Cell Survival / drug effects
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Cisplatin / pharmacology
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Colonic Neoplasms / pathology
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Colonic Neoplasms / surgery
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Coloring Agents
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Doxorubicin / pharmacology
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Drug Screening Assays, Antitumor
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Esophageal Neoplasms / pathology
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Esophageal Neoplasms / surgery
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Fluorouracil / pharmacology
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Gastrointestinal Neoplasms / pathology*
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Gastrointestinal Neoplasms / surgery
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Humans
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Leiomyosarcoma / pathology
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Leiomyosarcoma / surgery
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Liver Neoplasms / pathology
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Liver Neoplasms / surgery
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Mitomycin / pharmacology
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Rectal Neoplasms / pathology
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Rectal Neoplasms / surgery
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Stomach Neoplasms / pathology
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Stomach Neoplasms / surgery
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Tetrazolium Salts*
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Thiazoles*
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Tumor Cells, Cultured
Substances
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Antineoplastic Agents
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Coloring Agents
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Tetrazolium Salts
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Thiazoles
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Mitomycin
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Doxorubicin
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thiazolyl blue
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Cisplatin
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Fluorouracil