Maintenance of amelogenin gene expression by transformed epithelial cells of mouse enamel organ

Arch Oral Biol. 1992 Oct;37(10):771-8. doi: 10.1016/0003-9969(92)90110-t.

Abstract

Electroporation was used to introduce foreign genes into cells derived from the mouse enamel organ epithelia (EOE). Optimal conditions for this electroporation were established. The introduction of a plasmid construct bearing the coding region for the large T-antigen from polyoma virus into EOE cells permitted the establishment of a derivative cell line that has the following characteristics: (1) the cells could be passaged many times; (2) they expressed a keratin-containing cytoskeleton; and (3) approx. 60% of the cells expressed amelogenin, a tissue-specific gene product unique to ameloblasts. Potential uses for such a cell line include analysis of: (1) the upstream regulatory regions required for temporally and spatially restricted expression of amelogenin; (2) the post-translational modification of amelogenin in synchronized cells and (3) the organization and biomineralization of enamel extracellular matrix in monolayer culture.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amelogenin
  • Animals
  • Antigens, Viral, Tumor / genetics
  • Cell Line, Transformed
  • Dental Enamel Proteins / genetics*
  • Enamel Organ / cytology*
  • Epithelial Cells
  • Gene Expression*
  • Mice
  • Plasmids
  • Transfection / methods

Substances

  • Amelogenin
  • Amelx protein, mouse
  • Antigens, Viral, Tumor
  • Dental Enamel Proteins